PNGase F (Glycerol-free) from New England Biolabs

Product Specs
ItemPNGase F (Glycerol-free)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP0705L
Quantity75000 units
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature4 C
TypeGlycosidases

Specifications/Features

Storage:
Storage Conditions:
20 mM Tris-HCl
50 mM NaCl
5 mM Na2EDTA
pH 7.5 @ 25°C

Storage Temperature: 4°C
Store at 4°C, do not freeze.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Bivalent non-human gal-α1-3-gal glycan epitopes in the Fc region of a monoclonal antibody model can be recognized by anti-Gal-α1-3-Gal IgE antibodiesmAbsDecember 31, 2023Grayson Hatfield, Lioudmila Tepliakova, Jessica Tran, Huixin Lu, Michel Gilbert, Roger Y. Tamtransglycosylation reactions, remodeled glycans were characterized using PNGase F (New England Biolabs, Cat # P0705S) to release glycans, followed by HPAEC-PAD analysis as detailed below. For analysis using capillary electrophoresis

(2) Infection pressure in apes has driven selection for CD4 alleles that resist lentivirus (HIV/SIV) infectionbioRxivNovember 18, 2023Cody J. Warren, Arturo Barbachano-Guerrero, Vanessa L. Bauer, Alex C. Stabell, Obaiah Dirasantha, Qing Yang, Sara L. Sawyermanufacturer’s protocol, including a paired sample with no glycosidase as control (New England Biolabs, #P0705S). Treated whole cell extracts (5 μg per lane) were resolved on a 12% TGX Stain-free polyacrylamide gel

(3) False-Positive Glycopeptide Identification via In-FAIMS FragmentationJACS AuSeptember 25, 2023Valentina Rangel-Angarita, Keira E. Mahoney, Catherine Kwon, Raibat Sarker, Taryn M. Lucas, Stacy A. MalakerAmBic. After digestion, the first aliquot was further treated with 10 U of PNGaseF (New England BioLabs, P0705S) for 8 h at 37 °C. Protein Mixture The protein mixture was created by combining equal masses (5 μg)

(4) A Novel Inactive Isoform with a Restored Reading Frame Is Expressed from the Human Interferon Lambda 4 TT Allele at rs368234815Journal of Interferon & Cytokine ResearchSeptember 1, 2023Seema Bharatiya, Aditya Agarwal, Sreedhar ChinnaswamyChemiDoc MP imaging system (Bio-Rad). Deglycosylation Deglycosylation was done by using PNGase F (NEB; P0705S). For deglycosylation, 9 μL of cell lysate along with 1 μL of 10 × denaturing buffer was heated at 100°C

(5) Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityJournal of Biological ChemistryJune 1, 2023Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. Bertozziwith ammonium bicarbonate buffer and incubated at 37 °C with 2 μl of PNGase F (New England Biolabs P0705S) diluted 1:100 in ammonium bicarbonate for an additional 7 h. The sample was combined with 69 μl of

(6) Transcriptomic and glycomic analyses highlight pathway-specific glycosylation alterations unique to Alzheimer’s diseaseScientific ReportsMay 15, 2023Xinyu Tang, Jennyfer Tena, Jacopo Di Lucente, Izumi Maezawa, Danielle J. Harvey, Lee-Way Jin, Carlito B. Lebrilla, Angela M. Zivkovicusing 2 µL (500,000 units/mL) of peptide N-glycosidase F (glycerol-free; New England BioLabs, cat. no. P0705L). Ultracentrifugation at 200,000×g was used to separate the N-glycans and the membrane fraction. The

(7) Evolving spike-protein N -glycosylation in SARS-CoV-2 variantsbioRxivMay 12, 2023Sabyasachi Baboo, Jolene K. Diedrich, Jonathan L. Torres, Jeffrey Copps, Bhavya Singh, Patrick T. Garrett, Andrew B. Ward, James C. Paulson, John R. YatesCalcium chloride,Sigma-Aldrich,Cat#C5670 Proteinase K,Sigma-Aldrich,Cat#P2308 Trypsin,Promega,Cat#V5111 PNGase F,New England Biolabs,Cat#P0705 Ammonium bicarbonate ≥99.5%,Sigma Aldrich,CAT#09830 H218O (97% 18O),Sigma-Aldrich,Cat#329878 "Resin,

(8) Germline-targeting HIV-1 Env vaccination induces VRC01-class antibodies with rare insertionsCell Reports MedicineApril 18, 2023Tom G. Caniels, Max Medina-Ramírez, Jinsong Zhang, Anita Sarkar, Sonu Kumar, Alex LaBranche, Ronald Derking, Joel D. Allen, Jonne L. Snitselaar, Joan Capella-Pujol, et al.D2438 Acetic acid,Fisher Scientific,Cat# 10384970 Peptide-N-glycosidase F,New England Biolabs,Cat# P0705S Endoglycosidase H,New England Biolabs,Cat# P0702S Mass spectrometry grade trypsin,Promega,Cat# V5280 Sequencing

(9) Differentially expressed glycoproteins in pre‐ and post‐digital rectal examination urine samples for detecting aggressive prostate cancerPROTEOMICSApril 1, 2023Yuefan Wang, Tung‐Shing Mamie Lih, Naseruddin Höti, Lori J. Sokoll, Gregory Chesnut, Gyorgy Petrovics, Indu Kohaar, Hui Zhangwere purchased from Promega (Madison, WI, USA). PNGase F was purchased from New England Biolabs (# P0705L, Ipswich, MA, USA). Indexed retention time (iRT) standard peptides were from Biognosys Inc (Zurich,

(10) Loosenin-Like Proteins from Phanerochaete carnosa Impact Both Cellulose and Chitin Fiber NetworksApplied and Environmental MicrobiologyJanuary 31, 2023Mareike Monschein, Eleni Ioannou, Taru Koitto, Leamon A. K. M. Al Amin, Jutta J. Varis, Edward R. Wagner, Kirsi S. Mikkonen, Daniel J. Cosgrove, Emma R. Masterwere obtained from Sigma-Aldrich, PNGase F was from New England Biolabs (Ipswich, MA, USA; catalog no. P0705S), and endo-1,4-β-d-glucanase (cellulase from Trichoderma longibrachiatum, glycoside hydrolase family

(11) Human immunoglobulin repertoire analysis guides design of vaccine priming immunogens targeting HIV V2-apex broadly neutralizing antibody precursorsImmunityNovember 8, 2022Jordan R. Willis, Zachary T. Berndsen, Krystal M. Ma, Jon M. Steichen, Torben Schiffner, Elise Landais, Alessia Liguori, Oleksandr Kalyuzhniy, Joel D. Allen, Sabyasachi Baboo, et al.P0702 Calcium chloride,Sigma-Aldrich,Cat# C5670 Proteinase K,Sigma-Aldrich,Cat# P2308 PNGase F,NEB,Cat# P0705 Ammonium bicarbonate ≥99.5%,Sigma Aldrich,Cat# 09830 H218O (97% 18O),Sigma-Aldrich,Cat# 329878 "Resin,

(12) Galactosylceramidase deficiency and pathological abnormalities in cerebral white matter of Krabbe diseaseNeurobiology of DiseaseNovember 1, 2022Diego Iacono, Shunsuke Koga, Hui Peng, Arulmani Manavalan, Jessica Daiker, Monica Castanedes-Casey, Nicholas B. Martin, Aimee R. Herdt, Michael H. Gelb, Dennis W. Dickson, et al.performed following instructions from the PNGase F kit purchased from New England Biolabs, Ipswich, MA (P0705). Briefly, 20 μg of total protein from tissue supernatant was first denatured in 1× denaturation buffer

(13) Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffiiMicrobial Cell FactoriesSeptember 5, 2022Neil C. Dalvie, Christopher A. Naranjo, Sergio A. Rodriguez-Aponte, Ryan S. Johnston, J. Christopher Love40-80ug of total protein was digested with PNGaseF, glycerol-free (New England BioLabs, catalog no. P0705L) according to the manufacture’s recommended protocol. Intact mass analysis was performed on a 6530B

(14) Profiling the Bisecting N-acetylglucosamine Modification in Amniotic Membrane via Mass SpectrometryGenomics, Proteomics & BioinformaticsAugust 1, 2022Qiushi Chen, Yuanliang Zhang, Keren Zhang, Jie Liu, Huozhen Pan, Xinran Wang, Siqi Li, Dandan Hu, Zhilong Lin, Yun Zhao, et al.trypsin (V5111) was purchased from Promega (Madison, WI). Peptide N-glycosidase F (PNGase F; Catalog No. P0705L) was purchased from New England Biolabs (Beijing, China). Methanol (Catalog No. A412-4), acetonitrile

(15) HEPES‐buffering of bicarbonate‐containing culture medium perturbs lysosomal glucocerebrosidase activityJournal of Cellular BiochemistryMay 1, 2022Martijn J. C. van der Lienden, Jan Aten, Rolf G. Boot, Marco van Eijk, Johannes M. F. G. Aerts, Chi‐Lin Kuocolumn (Pierce; 89849) and incubated with PNGase F according to the manufacturer's instructions (NEB; P0705S). Shortly, denaturation of protein was performed in denaturing buffer at 100°C for 10 min. Subsequent

(16) Distinctive Roles of Furin and TMPRSS2 in SARS-CoV-2 InfectivityJournal of VirologyApril 27, 2022Rachid Essalmani et al.were digested for 90 min at 37°C with endoglycosidase H (endo-H; P0702L) or endoglycosidase F (endo-F; P0705S) as recommended by the manufacturer (New England BioLabs). Inhibitor treatment. At 24 h posttransfection,

(17) On-tissue spatially resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscapeCell Chemical BiologyJanuary 1, 2022Stacy Alyse Malaker, Jusal Quanico, Antonella Raffo-Romero, Firas Kobeissy, Soulaimane Aboulouard, Dominique Tierny, Carolyn Ruth Bertozzi, Isabelle Fournier, Michel SalzetFL-1301-2 Sequence grade modified trypsin,Promega,V5111 Glycerol free PNGaseF,New England Biolabs,P0705S "Ethanol, 200 proof",Fisher,CAS 64-17-5 Pierce Ultrapure Water,ThermoFisher,Cat# 51140 Deposited

(18) Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptoreLifeDecember 20, 2021Tümay Capraz et al.probe,FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1 Other,FreeStyle F17 medium,Thermo Fisher Scientific,Cat #: A1383502, Other,PNGase F,New England BioLabs,Cat #: P0705,"180,000 U mL–1" Other,Neuraminidase,New England BioLabs,Cat #: P0720,"2,500 U mL–1" "Chemical compound,

(19) The SARS CoV-2 spike directed non-neutralizing polyclonal antibodies cross-react with Human immunodeficiency virus (HIV-1) gp41International ImmunopharmacologyDecember 1, 2021Reshma Perween et al.dilution was kept stable at 1:2000. 2.8 Protein’s enzymatic de-glycosylation: PNGase F (NEB, Cat. No P0705S) (Non-Denaturing Reaction Conditions) was used to deglycosylate the protein in accordance with the manufacturer's

(20) Non-neutralizing SARS CoV-2 directed polyclonal antibodies demonstrate cross-reactivity with the HA glycans of influenza virusInternational ImmunopharmacologyOctober 1, 2021Praveenkumar Murugavelu et al.Protein’s enzymatic de-glycosylation: Protein de-glycosylation was performed by PNGase F (NEB, Cat. No P0705S) (Non-Denaturing Reaction Conditions) and Endo H (NEB, Cat. No P0702S) following the manufacturer’s

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